From Conventional Methods to Innovation: Caffeine and Chlorogenic Acid Extraction and Quantification in the Rise of Smart and Green Techniques—A Systematic Review

Two molecules in your morning coffee are quietly shaping the future of green chemistry—one keeps you awake, the other fights oxidative stress. A new systematic review reveals how scientists are swapping old-school solvents for smarter, eco-friendly extraction methods.

Caffeine and chlorogenic acid are the dynamic duo of coffee and tea, celebrated for their roles in boosting alertness and offering antioxidant, anti-inflammatory, and metabolic benefits. But getting them out of plants and measuring them accurately is no small feat. For decades, conventional solvent-based extraction has been the go-to approach—effective, but not exactly green. Now, a systematic review following PRISMA guidelines has taken stock of the field, sifting through 125 studies to map the landscape of extraction and quantification methods across diverse plant matrices.

Key Points

• 125 studies systematically reviewed following PRISMA guidelines.
• Green methods like ultrasound and microwave extraction are rising.
• HPLC with UV or MS remains the quantification benchmark.