Green and Efficient Extraction of Soybean (Glycine max (L.) Merr.) Isoflavones Using Natural Deep Eutectic Solvents-Based Ultrasound-Assisted Extraction

Imagine a greener way to unlock the health-boosting secrets of soybeans—using a solvent that’s as natural as the beans themselves. This new method not only boosts yield but also slashes environmental harm.

Soybeans are a global food powerhouse, packed with isoflavones—bioactive compounds known for their antioxidant, anti-inflammatory, and phytoestrogenic benefits. But extracting these treasures has always been a double-edged sword: conventional solvents often lack selectivity, struggle with glycosylated forms, and leave a heavy environmental footprint. Now, researchers have turned to Natural Deep Eutectic Solvents (NADES) paired with ultrasound assistance, a combo that’s both green and efficient.

Key Points

• NADES-based UAE yields 1076.78 µg/g DW isoflavones.
• Choline chloride-ascorbic acid (1:1) outperforms 50% ethanol.
• Optimized at 39% water, 55°C, 64 min; hydrogen bonding key.