Optimization of the effect of cold plasma treatment on UAE-NADES green extraction of chickpea roots (Cicer arietinum) bioactive compounds

What if the secret to unlocking hidden bioactive treasures lies in a burst of cold plasma? Chickpea roots, often discarded, are getting a high-tech makeover.

Chickpea roots are an overlooked agricultural by-product, but they hold a wealth of bioactive compounds. A new study introduces a clever two-step extraction method to tap into this potential. First, the root powder is treated with atmospheric air low-pressure cold plasma—a non-thermal, eco-friendly technology. Then, ultrasound-assisted extraction with natural deep eutectic solvents (NADES) takes over, maximizing the yield of valuable antioxidants and phenolic compounds.

The researchers optimized the plasma conditions using response surface methodology, zeroing in on 11.5 minutes, 52 watts, and 0.65 mbar. This combined approach significantly boosted total phenolic content and antioxidant activity compared to untreated samples. The Box-Behnken design predicted values that matched experimental results, confirming the method's reliability.

Beyond extraction efficiency, the study also assessed microbial load reduction and color stability, ensuring the treated roots maintain quality and functionality. This sustainable, green methodology offers a promising way to valorize chickpea roots for use in functional foods, nutraceuticals, and pharmaceuticals—turning waste into wellness.

Key Points

• Cold plasma + UAE-NADES boosts chickpea root bioactives.
• Optimal conditions: 11.5 min, 52 W, 0.65 mbar.
• Sustainable valorization for food and pharma.