RSM- and ANN-Based Optimization of Bioactive Anthocyanin and Phenolic Compound Recovery from Rosa damascena Flowers Using Natural Deep Eutectic

What if the secret to unlocking the full potential of the Damask rose lies not in a high-tech lab, but in a simple, eco-friendly solvent? A new study reveals how natural deep eutectic solvents can turn these petals into a powerhouse of antioxidants and antimicrobials.

Deep inside the petals of the Damask rose lies a treasure trove of health-boosting compounds, but extracting them has always been a challenge. Now, researchers have found a greener way: using natural deep eutectic solvents (NADES) combined with ultrasound. These solvents, made from natural ingredients, are not only environmentally friendly but also highly effective at pulling out anthocyanins and phenolic compounds.

The study screened a variety of these solvents and identified choline chloride–propylene glycol as the star performer. To fine-tune the extraction, the team used two powerful optimization tools: response surface methodology (RSM) and artificial neural networks (ANN). Under the optimal conditions, the extracts boasted impressive levels of bioactive compounds, including anthocyanins and phenolics, alongside strong antioxidant activity.

But the benefits don't stop there. The extracts also showed antimicrobial activity against foodborne pathogens, and their stability was remarkable—especially when stored in the dark at cooler temperatures. This research paves the way for a sustainable, efficient method to harness the full potential of Damask roses, from natural colorants to functional food ingredients.

Key Points

• NADES-UAE efficiently recovers anthocyanins and phenolics from Damask rose.
• Optimal conditions yield high bioactive content and antioxidant activity.
• Extracts show antimicrobial effects and stability in dark, cool storage.